The majority of secondary antibodies against antibodies from common research models such as mouse or rat are polyclonal antibodies made in goats or rabbits. These not only suffer from reproducibility issues, but also come with a considerable impact on animal welfare. At Absolute Antibody, all our antibodies, including our secondary antibodies, are manufactured in a recombinant, completely animal- and animal-component free production platform. We are able to offer recombinant anti-immunoglobulin antibodies against mouse or rat antibodies, as well as antibodies that bind rabbit, goat, dog, and elasmobranch (e.g. sharks, rays, skates) antibodies.
Anti-immunoglobulin antibodies are used as secondary antibodies to detect primary antibodies in methods such as western blot, immunohistochemistry, and immunofluorescence staining for microscopy or flow cytometry. Recombinant production provides highly reliable anti-immunoglobulin antibodies with the highest possible batch-to-batch reproducibility. In addition, recombinant antibody technology enables antibody engineering to open up new experimental possibilities.