United Kingdom (UK)
Recombinant monoclonal antibody to IgG1 (hinge). Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody TP885.
Alternative Name(s) of Target: immunoglobulin G1
Immunogen: This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Specificity: This antibody recognises mouse and rat IgG1, with a proposed epitope located at the hinge region.
Application Notes: TP885 nanobody can be used for the purification of antigens or antigen complexes previously bound to a primary antibody, as it successfully isolated mouse monoclonal IgG1 from hybridoma cell culture supernatant and then released it under physiological conditions using SUMOStar protease cleavage (Pleiner et al., 2018).
Antibody first published in:
Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID:29263082
Note on publication:
The article describes the generation and characterisation of this nanobody.