Antibody Protein Sequencing

There are many monoclonal antibodies for which the hybridoma cell line is unavailable. To complement our hybridoma sequencing service, we are also offering a de novo antibody protein sequencing service.

By combining mass-spectrometry with high-accuracy de novo peptide sequencing and assembly of overlapping peptides, the amino acid sequences of the variable domains are determined.

We guarantee to deliver a functional antibody sequence along with a comprehensive sequencing report with full sequence coverage. In combination with our transient expression service, we

MS_workflow

De novo sequencing workflow: The antibody is digested using multiple enzymes. On each digest LC-MS-MS is performed and peptides predicted de novo. Using proprietary software, overlapping peptides are assembled into a full coverage protein sequence.

can sequence an antibody and deliver recombinant versions in as little as 9 weeks.

The whole combined service is priced at 13,600 GBP / 17,500 USD / 15,300 EUR. For more information please contact us.

Customer provides Mass Spectrometric Sequencing Antibody Expression Timeline Deliverables
Monoclonal antibody, >0.2 mg, 95% pure
  • Enzymatic digest into overlapping peptides
  • LC-MS/MS using Thermo Orbitrap
  • De novo identification of peptides
  • Automated peptide assembly
  • Curation of results by experienced scientists
  • Gene synthesis
  • Cloning
  • Transient expression in HEK293 cells
  • Affinity purification
  • Buffer exchange (PBS)
  • Sterile filtration
  • Purity >98% (SDS-PAGE)
  • Endotoxin < 1EU/mg
9 weeks
  • Comprehensive sequencing report
  • 3 mg purified antibody
  • QC data