Hexpress - Guaranteed antibody expression service

To meet the growing demands for recombinant antibodies across research and diagnostics Absolute Antibody have launched the HEXpress service. A proprietary transient expression system for rapid manufacturing of milligram to gram quantities of antibody in mammalian HEK293 cells. A faster more affordable alternative to stable CHO cell line generation with higher quality and flexibility than traditional hybridoma production. Simply provide us with an electronic sequence or hybridoma cell line and we’ll take care of the rest. We’ll even guarantee the quantity and quality of the antibody for all your in vitro or in vivo needs.

We offer two levels of service, Silver and Gold. Both provide highly purified antibody suitable for all purposes. The Gold service offers tighter control on monomer purity through removal of trace amounts of aggregate.

If you want to express a particular antibody but don’t have the sequence yet, please contact us and we will be happy to assist you.

Hexpress – Silver service

The HEXpress Silver service offers high quality recombinant antibody expression at affordable prices. The service covers gene synthesis to highly purified antibody in as little as 6 weeks.

Customer provides Scale and format Standard service details Timeline Deliverables
Electronic sequence or hybridoma 1 mg to 2 grams
Any IgG species or subtype
  • Gene synthesis
  • Cloning
  • Affinity purification
  • Buffer exchange (PBS)
  • Sterile filtration
  • Purity >98% (SDS-PAGE)
  • Endotoxin < 1EU/mg
6-8 weeks
  • Purified antibody
  • QC data

Academic discount: Absolute Antibody offer a 10% discount to academic institutions on all custom services.

For further information or pricing of the HEXpress Silver service please contact us and include details of the number, quantity and type of antibody you want expressing.

If you have a hybridoma the HEXpress silver service can be combined with our hybridoma sequencing service.

Hexpress – Gold service

The HEXpress Gold service has been developed for those in pre-clinical development of therapeutic antibodies. This service provides the same speed as the standard service but with stricter control on monomer purity and endotoxin levels.

 

Customer provides Scale and format Premium service details Timeline Deliverables
Electronic sequence or hybridoma 1mg to 2 gramsAny IgG species or subtype
  • Turbo gene synthesis
  • Cloning
  • Affinity purification
  • Size exclusion chromatography
  • Sterile filtration
  • Monomer purity >98% (HPLC)
  • Endotoxin <0.5 EU/mg
5-8 weeks
  • Purified antibody
  • QC data

For further information or pricing of the HEXpress premium service please contact us and include details of the number, quantity and type of antibody you want expressing.

If you have a hybridoma the HEXpress Gold service can be combined with our hybridoma sequencing service.

Hexpress – Options

The HEXpress services can be applied to IgGs of any subtype from any species. All formats shown in the table below are already available at Absolute Antibody. Please contact us if you want an IgG from a species that is not shown and it is highly likely we can deliver this for you.

Human Mouse Rat Armenian Hamster Rabbit Goat Rhesus Macaque
Available IgGs IgG1/2/3/4 IgG1/2a/2b/2c/3 IgG1/2a/2b/2c IgG IgG IgG IgG1

We also have the ability to generate a range of antibody fragments and other formats. See our Antibody Engineering page for a list of the formats that are available or contact us to discuss your exact requirements.

Have you already got your own antibody expression vector(s)? It is highly likely we can express directly from your vector(s) and remove the need for gene synthesis and cloning. Contact us directly to discuss this option and provide us with details of your vector(s) – promotor, signal peptide, separate heavy and light chain vectors or a single vector, size, antibiotic resistance etc.

Hexpress – Guarantee

Absolute Antibody will guarantee the agreed quantity and quality of the antibody produced. The customer will incur no charge if these criteria are not met. Timelines shown are representative of the average timelines for this service.

Variant Expression

Absolute Antibody offers expression and purification of panels of up to 50 antibodies at one time at a scale of 1-2mg. This may be of particular use for those wishing to analyse variants in an antibody screening or optimisation campaign.

For further information please do not hesitate to contact us.