Murine Bispecific Antibody Reagents

Bispecific antibodies are an exciting class of therapeutics permitting simultaneous engagement of two distinct targets with the same antibody. Knob-into-hole (KIH) bispecific antibodies are playing an important role in drug development, as this pioneering format enables heavy-chain heterodimerization and the creation of an IgG-like antibody with different targets on each arm. However, researchers have lacked syngeneic mouse models for evaluating KIH bispecific combinations, as the mutations applied to human IgG1 cannot be readily transferred to murine IgG subtypes.

To address this, Absolute Antibody has developed the first commercially available platform for the generation of fully murine KIH bispecific antibodies. Customers can purchase bispecific antibodies from our catalog off-the-shelf, or mix-and-match targets from the catalog to build their own custom reagent, to enable easier evaluations of potential bispecific combinations in mouse models.

Highlights of our murine KIH bispecific antibodies include:

Our anti-mCD3ε:TRP-1 bispecific antibody led to effective killing of transgenic TRP-1+ tumor cells. Download our research poster to learn more.

  • One IgG ‘arm’ uses a single-domain binder to overcome the light-chain pairing problem
  • Our proprietary Fc Silent™ domain abrogates Fc gamma receptor and C1q binding without affecting FcRn-mediated recycling
  • Fully murine backbone for low immunogenicity in mouse models
  • Low endotoxin levels and high purity for in vivo research
  • Our T-cell-engaging CD3ε-bispecific antibodies have demonstrated success
    killing tumor cells in mouse models

For more information, download our research poster to learn about the generation of our KIH bispecific antibody platform and review functional characterization of our anti-mCD3ε:TRP-1 bispecific antibody, capable of selectively recruiting T-cells to TRP-1+ cancer cells for increased cytotoxic effector function. You can also read a customer case study about researchers at the Leiden University Medical Center who used two of our bispecific antibodies in a study that showed oncolytic viruses in combination with CD3ε-bispecific antibodies could improve the immunotherapy treatment of solid cancer tumors.

In addition to our KIH bispecific antibodies, further murine bispecific antibody constructs are available in an IgG-dAb format, enabling bivalent engagement of each target and representing further antibody engineering options available to our customers. To learn more about our suggested initial go-to formats for bispecific antibodies, as well as the utility of mouse bispecifics in basic research and drug discovery, view our presentation here.

View our off-the-shelf bispecific antibodies below, and follow the links to place your order. Please contact us to discuss creating a custom bispecific reagent, using either clones from our catalog or your own antibodies. Starting with a hybridoma cell line, purified antibody or antibody sequence, we can deliver milligram-to-gram quantities of your custom bispecific, 100% royalty-free.

Available Bispecific Antibodies:

herbB-2 (Her-2/neu)
mVISTA (PD-1H)mIgG-dAb