United Kingdom (UK)
Recombinant monoclonal antibody to bicyclo[2.2.2]octene hapten 4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from 39-A11.
Specificity: The antibody binds to hapten 4 with an affinity (Kd) of 10 ± 0.3 nM. The antibody has a Kcat of 0.67 s−1 and a Km of 1200 μM.
Application Notes: The antibody binds specifically to the bicyclo[2.2.2]octen moiety of hapten 4, and catalyses the pericyclic Diels- Alder reaction. The Diels-Alder is an organic chemical reaction between a conjugated diene and a dienophile which results in the formation of a substituted cyclohexene system. The Monoclonal antibody 39-A11 binds to the diene and dienophile in a reactive orientation, thereby catalysis the reaction by lowering the activation energy. The antibody has high affinity for hapten 4, and low affinity for the reaction end product, allowing it to be released once the reaction has terminated.
Antibody first published in:
Romesberg FE, Spiller B, Schultz PG, Stevens RC. Immunological origins of binding and catalysis in a Diels-Alderase antibody. Science. 1998 Mar 20;279(5358):1929-33. PMID:9506942
Note on publication:
Describes the generation and characterization of the three-dimensional structure of the monoclonal antibody 39-A11, a catalysis in the Diels-Alder reaction.