Anti-bicyclo[2.2.2]octene hapten 4 [39-A11] Standard size

Description:
Isotype and Format: Mouse IgG1, kappa
Clone Number: 39-A11
Published Application(s): ELISA
Published Species Reactivity:
Immunogen:
Specificity: The antibody binds to hapten 4 with an affinity (Kd) of 10 ± 0.3 nM. The antibody has a Kcat of 0.67 s-1 and a Km of 1200 μM.
Application Notes: The antibody binds specifically to the bicyclo[2.2.2]octen moiety of hapten 4, and catalyses the pericyclic Diels- Alder reaction. The Diels-Alder is an organic chemical reaction between a conjugated diene and a dienophile which results in the formation of a substituted cyclohexene system. The Monoclonal antibody 39-A11 binds to the diene and dienophile in a reactive orientation, thereby catalysis the reaction by lowering the activation energy. The antibody has high affinity for hapten 4, and low affinity for the reaction end product, allowing it to be released once the reaction has terminated.
Antibody First Published in:
Romesberg FE, Spiller B, Schultz PG, Stevens RC. Science. 1998 Mar 20;279(5358):1929-33. PMID:9506942 Note on publication: Describes the generation and characterization of the three-dimensional structure of the monoclonal antibody 39-A11, a catalysis in the Diels-Alder reaction.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00509-1.1

Mouse IgG1

Ships in 4-6 weeks
Standard size 200 µg
Custom conjugation:
Clear selection
Ships in 4-6 weeks
Bulk size
1 mg

£1,100