United Kingdom (UK)
Recombinant monoclonal antibody to OX40L. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-89.
UniProt Accession Number of Target Protein: P43488
Alternative Name(s) of Target: CD252; Tumor necrosis factor ligand superfamily member 4; OX40 ligand; CD134 ligand; OX40R
Immunogen: This antibody was prepared by immunizing PVG rats with a recombinant chimeric protein consisting of domains 3 and 4 of rat CD4 and the extracellular region of mouse CD134L.
Specificity: This antibody is specific for murine CD252 (the ligand for OX40), expression of which is restricted to activated B cells, dendritic cells, microglia and endothelial cells.
Application Notes: This antibody binds mouse CD134L, and has been shown to bind activated B cells and DCs, as well as CD134L-transfected fibroblast cells, though not activated T cells, in labelling and immunohistochemical analysis (Malmström, 2001). In addition, this antibody is able to partially block the interaction between CD134 and CD134L. This blocking effect could be responsible for the ability of this antibody to prevent T cell accumulation in the intestine of T cell-restored SCID mice and inhibits the development of Th1-mediated colitis.
Antibody first published in:
Malmström et al. CD134L Expression on Dendritic Cells in the Mesenteric Lymph Nodes Drives Colitis in T Cell-Restored SCID Mice J Immunol. 2001 Jun 1;166(11):6972-81. PMID:11359859
Note on publication:
Describes the original use of this antibody in blocking studies to implicate CD134L expression on DCs in mouse mesenteric lymph nodes in the development of colitis.
Immunofluorescence staining of fixed mouse splenocytes with anti-OX40L (Tumor necrosis factor ligand superfamily member 4) antibody OX-89 (Ab00564) Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of OX-89 (Ab00564-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00564-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.