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- Ab00609-1.1 Anti-EpCAM [AUA1]
- Human
- Mouse IgG1
- Purified
- In Stock
- Ab00609-10.0 Anti-EpCAM [AUA1]
- Human
- Human IgG1
- Purified
- Ships in 4-5 weeks
- Ab00609-23.0 Anti-EpCAM [AUA1]
- Human
- Rabbit IgG
- Purified
- Ships in 4-5 weeks
Recombinant monoclonal antibody to EpCAM. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AUA1.
UniProt Accession Number of Target Protein: P16422
Alternative Name(s) of Target: CD326; Epithelial Cell Adhesion Molecule
Immunogen: LoVo cell line, a colon carcinoma cell line.
Specificity: This antibody binds human EpCAM.
Application Notes: AUA1 cross reacts with breast carcinomas, kidney tubules, tonsil crypt epithelium and cells in some serious effusions. This is a good antibody for characterisation of all epithelial cells.
Antibody first published in: PMID:
Immunofluorescence staining of fixed Caco-2 cells with anti-EpCAM antibody AUA1 (Ab00609) Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of AUA1 (Ab00609-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing strong membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00609-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.