United Kingdom (UK)
Recombinant monoclonal antibody to IFN-gamma. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from phage display 6D7.
UniProt Accession Number of Target Protein: A0SDD7
Alternative Name(s) of Target: IFN-g; Interferon gamma; Type II interferon; IFN-gamma; IFN-y; IFN-g
Immunogen: Chicken antibodies were produced by immunising Hy-Line silver brown chickens with recombinant white rhino IFN-gamma. The spleens of immunised chickens were collected and total RNA was reverse transcribed into cDNA.
Specificity: scFv 6D7 reacted with both white rhino (Ceratotherium simum) and Asian elephant (Elephas maximus) IFN-gamma, shown by direct ELISAs.
Application Notes: 6D7 has been used as a capture antibody in ELISAs testing for the presence of IFN-gamma.
Antibody first published in:
Morar et al. Cloning, sequencing and expression of white rhinoceros (Ceratotherium simum) interferon-gamma (IFN-gamma) and the production of rhinoceros IFN-gamma specific antibodies. Vet Immunol Immunopathol. 2007 Jan 15;115(1-2):146-54. PMID:17118460
Note on publication:
Describes the generation of murine and chicken antibodies against white rhino IFN-gamma and their use in the development of a diagnostic assay for the detection of tuberculosis.