IgG Antibody Production

We developed our proprietary HEXpress™ antibody expression platform to meet the growing demand for recombinant antibody technology across pharmaceuticals, biotechnology, diagnostics and academia. This serum-free mammalian transient expression system offers a faster, more affordable alternative to stable cell line generation, rapidly producing recombinant antibodies with high purity and low endotoxin levels.

While Absolute Antibody has manufactured more than 140 different antibody formats to date – including multispecifics, fragments and other novel formats – many of our clients require standard IgG antibody production. We manufacture IgG antibodies in any species or subtype (including chimeric antibodies) at milligram-to-gram quantities, starting from your electronic antibody sequence, hybridoma cell line or purified antibody protein. Our ISO 9001:2015-certified manufacturing facility provides high enough production capacity for any project, with the ability to scale up to multi-gram quantities within weeks.

We offer two levels of antibody production, HEXpress™ Silver and Gold, which both provide highly purified antibody suitable for in vitro and in vivo needs. HEXpress™ Gold offers stricter control on monomer purity and endotoxin levels for therapeutic antibody development. Read about each service below. To learn more, contact us or fill out the form below to start a discussion about your project.

We also offer other isotypes and engineered antibody production, as well as a dedicated high-throughput antibody production service, if you have other antibody expression needs.

HEXpress™ Silver Antibody Expression Service

The HEXpress™ Silver service offers high-quality recombinant antibody expression at affordable prices, with our academic customers receiving a further 10% discount. The service covers gene synthesis to highly purified antibody in as little as 6 weeks.

Customer provides Scale and format Standard service details Timeline Deliverables
Electronic sequence or hybridoma 1 mg to 2 grams
Any IgG species or subtype
  • Gene synthesis
  • Cloning
  • Affinity purification
  • Buffer exchange (PBS)
  • Sterile filtration
  • Purity >98% (SDS-PAGE)
  • Endotoxin < 1EU/mg
6-8 weeks
  • Purified antibody
  • QC data

HEXpress™ Gold Antibody Expression Service

The HEXpress™ Gold service has been developed for those in pre-clinical development of therapeutic antibodies. This service provides the same speed as the standard service but with stricter control on monomer purity and endotoxin levels.

Customer provides Scale and format Premium service details Timeline Deliverables
Electronic sequence or hybridoma 1 mg to 2 grams

Any IgG species or subtype

  • Cloning
  • Affinity purification
  • Size exclusion chromatography
  • Sterile filtration
  • Monomer purity >98% (HPLC)
  • Endotoxin <0.5 EU/mg
6-8 weeks
  • Purified antibody
  • QC data

For both the HEXpress™ Silver and Gold services, Absolute Antibody guarantees the quantity and quality of the antibody produced. If these criteria are not met, the customer will incur a 30% project initiation cost but no further costs. Timelines shown are representative of the average timelines for this service. Delays in delivery do not restrict Absolute Antibody’s right to invoice the full cost of the project.

CHXpress™ Antibody Expression

In 2021, we expanded our services to offer our transient CHXpress™ antibody expression platform. CHXpress™ is also available as a Silver or Gold service. With the addition of this service, we now offer serum-free mammalian transient expression in both HEK and CHO platforms, enabling us to better serve clients through their full antibody development pathway. Contact us via the form below to learn more.

Contact Us About Your Antibody Expression Project

Fill out the form below to start a conversation about your antibody expression needs. A sales representative will be in touch shortly to discuss details, review pricing and answer any questions.
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