United Kingdom (UK)
Recombinant monoclonal antibody to 8-OH-Adenosine. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 8A9.
Alternative Name(s) of Target: adenosine; hydroxyadenosine; hydroxy-adenosine; 8-hydroxy-adenosine; 8 hydroxy adenosine; Ade; 8-Ox-Ade
Immunogen: This antibody was raised by immunizing balb/c mice subcutaneously with 8-OH-adenosine conjugated to keyhole limpet hemocyanin.
Specificity: This antibody is specific for 8-OH-adenosine.
Application Notes: 8A9 is recommended for the detection of 8-OH-Adenosine. In an inhibition experiment it was tested wether a ligand for 8-OH-adenosine could inhibit the binding of this antibody to its antigen. It was shown that a concentration betweeen 1 and 5 um ihibits the antibody binding to its antigen (US6900291B2). The ability this antibody to detect 8-OH-Ade lesions in physiological DNA was tested. A dot blot was used to test this (US6900291B2). This antibody was tested for binding to 8-OH-adenosine conjugated BSA in an ELISA on 96-well Probind plates (US6900291B2).
Antibody first published in: PMID: