United Kingdom (UK)
Recombinant monoclonal antibody to Acetyllysine. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma ACK2F12.
Alternative Name(s) of Target: acetylated lysine; acetyl derivative of amino acid lysine; N-ε-acetyl-L-lysine
Immunogen: The original antibody was generated by immunizing mice with chemically acetylated keyhole limpet hemocyanin.
Specificity: This antibody recognizes acetly lysine and more particularly Nε-acetyllysine.
Application Notes: This antibody is capable of identifying acetylated BSA and various acetlylysine containing mouse peptides in an ELISA. This antibody can also recognize acetyllysine-containing protein from human T cell leukemia cell line MOLT-4F lysate in a western blot assay (EP1783142, ISBN-10: 1594545502). The HRP-SH conjugated ACK2F12 was capable of detecting chemically conjugated BSA in a western blot assay (EP1783142). This antibody was used in the study of organ specific post translational lyine acetylation in mice (PMID: 16247734)
Antibody first published in:
Iwabata et al. Proteomic analysis of organ-specific post-translational lysine-acetylation and -methylation in mice by use of anti-acetyllysine and -methyllysine mouse monoclonal antibodies. Proteomics. (2005); 5(18):4653-64. PMID:16247734
Note on publication:
Describes the analysis of organ-specific post-translational lysine-acetylation in mice using this antibody.