United Kingdom (UK)
Recombinant monoclonal antibody to Amyloid beta. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody scFv59.
UniProt Accession Number of Target Protein: P05067
Alternative Name(s) of Target: Aβ; Abeta; Amyloid-beta precursor protein; APP; ABPP; APPI; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide (CVAP); PreA4; Protease nexin-II (PN-II)
Immunogen: The original antibody was isolated from a human scFv phage library by screening against Abeta1-42.
Specificity: This antibody is specific for oligomeric as well as fibrillar/aggregated Aβ.
Application Notes: The scFv version of this antibody demonstrated the highest ELISA titer for Aβimmunoreactivity against Aβ1–42, and its specificity for oligomeric and fibrillar/aggregated Aβ was demonstrated in a WB. The scFv version of this antibody reacted with amyloid beta deposits in brain sections from a Tg2576 mouse and in skeletal muscle sections from a Tg13592 mouse, as observed via IHC. The scFv version of this antibody was used to inhibit the formation of Aβ fibril formation in vitro as demonstrated in a Thioflavin T assay. This inhibition contributed to the amelioration of Aβ cytotoxicity as was shown in vitro in an MTT assay; cell viability increased from 45.2 ± 0.78% to 58.8 ± 5.45% (P = 0.069) in the presence of scFv59. Furthermore, Tg2576 mice subjected to scFv59 injection in the hippocampus and cortex had fewer diffuse and fibrillar Aβ deposits in those areas than Tg2576 mice injected with PBS (Fukuchi et al., 2006; PMID: 16630540). Vascular amyloid was also stained by scFv59 (Fukuchi et al., 2006; PMID: 16766200).
Antibody first published in:
Fukuchi et al. Amelioration of amyloid load by anti-Abeta single-chain antibody in Alzheimer mouse model Biochem Biophys Res Commun. 2006 May 26;344(1):79-86. doi: 10.1016/j.bbrc.2006.03.145 PMID:16630540
Note on publication:
The original publication explores the potential of a human single-chain antibody that specifically targets oligomeric Abeta and amyloid plaques in Alzheimer's disease mouse models.