United Kingdom (UK)
Recombinant monoclonal antibody to Annexin XI. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the chicken B-cell clone A5A3.
UniProt Accession Number of Target Protein: P50995
Alternative Name(s) of Target: Annexin A11; 56 kDa autoantigen; Annexin-11; Calcyclin-associated annexin 50; CAP-50
Immunogen: The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
Specificity: The antibody is specific for human annexin XI. Annexin XI binds specifically to calcyclin in a calcium-dependent manner and it is required for midbody formation and completion of the terminal phase of cytokinesis.
Application Notes: The binding characterization of this antibody was done using ELISA. This antibody is also capable of recognizing human annexin XI in a western blot assay (Hof et al., 2008; PMID: 18062792).
Antibody first published in:
Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens Clin Exp Immunol. 2008 Feb;151(2):367-77. PMID:18062792
Note on publication:
Describes the generation of chicked antibodies against various human autoantigens.