Anti-Arginase 1 [mAb1]

Name: Anti-Arginase 1 [mAb1]
SKU: Ab03082
Availability: InStock

Description:

Recombinant monoclonal antibody to Arginase 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody mAb1.

Clone Name:

mAb1

Published Application(s):

Cryo-electron microscopy, inhibition

Published Species Reactivity:

Human

Products based on this clone:

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Ab03082-23.0 Anti-Arginase 1 [mAb1]
Human
Rabbit IgG
Purified
In Stock

Ab03082-2.0 Anti-Arginase 1 [mAb1]
Human
Mouse IgG2a
Purified
In Stock

UniProt Accession Number of Target Protein: P05089

Alternative Name(s) of Target: ARG1; Arginase-1; Liver-type arginase; Type I arginase
Immunogen: The antibody is a human antibody identified via yeast display technologies and isolated after panning against arginase 1.

Specificity: The antibody sterically blocks the arginase 1 active site near the exterior surface of the enzyme. Approximately 80% of all complexes identified were composed of two arginase 1 trimers and three antibodies, forming a 2:3 complex, while the remaining 20% of complexes were composed of two arginase 1 trimers and two antibodies; however, the epitope and paratope interactions between each antibody and arginase 1 monomers are identical. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.

Application Notes: The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of human arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki for the antibody against human Arg1 was 3.3 ± 0.3 nM (Palte et al., 2021; PMID: 34326456).

Antibody first published in: Palte et al. Cryo-EM structures of inhibitory antibodies complexed with arginase 1 provide insight into mechanism of action. Commun Biol. 2021 Jul 29;4(1):927. PMID:34326456
Note on publication: The paper describes the generation and characterization of the antibody. The antibody was used as probes for Human Arginase 1 conformational study.

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