United Kingdom (UK)
Recombinant monoclonal antibody to B7-H4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 6H3.
UniProt Accession Number of Target Protein: Q7Z7D3
Alternative Name(s) of Target: B7H4; B7 homolog 4; B7h.5; O8E; Immune costimulatory protein B7-H4; B7S1; Protein B7S1; B7x; T-cell costimulatory molecule B7x; VTCN1; V-set domain-containing T-cell activation inhibitor 1
Immunogen: The original antibody was raised by immunizing a DO11.10 mouse with B7-H4.
Specificity: This antibody is specific for the IgC region of human B7-H4.
Application Notes: Humanized variants of this antibody 6H3V6 and 6H3V7 are available on request. This antibody is able to neutralize the effect of an B7-H4 blocking antibody (US9574000). Flow cytometry was performed on HEK293 cells that expressed full length human B7-H4 using this antibody (US9574000). This antibody induces robust internalization of B7-H4 expressed on the surface of both the B7-H4 transfected cell line and the B7-H4 positive breast cancer cell line SK-BR-3 (US9574000). Immunohistochemistry was performed on human tissue using this antibody (US9574000). An internalization assay was performed using this antibody on EG7 (B7-H4 negative cells), EG7.IG7 (B7-H4 low expressing cells), and EG7.IVB3 (B7-H4 high expressing cells) (US9574000). Antibody-Dependent Cell-Mediated Cytotoxicity assay was performed on EG7.hB7-H4 (clone IVB3) cells and SK-BR-3 cells using this antibody (US9574000). An ELISA was performed on B7-H4ECD his-tagged protein using this antibody (US9574000).
Antibody first published in: Langermann et al. Anti-human B7-H4 antibodies and their uses. US9574000 PMID: