United Kingdom (UK)
Recombinant monoclonal antibody to C3b-iC3b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 7C12.
UniProt Accession Number of Target Protein: P01024
Alternative Name(s) of Target: Complement C3; C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
Immunogen: The mouse version of this antibody was raised by immunizing a mouse with human C3b and iC3b bound to a carbohydrate matrix via the alternative pathway of complement activation.
Specificity: This antibody is specific for human C3b. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Application Notes: To prepare and characterize this antibody, an ELISA on iC3 protein was performed using the mouse version of this antibody (Tosic et al, 1989; pmid:2786912). An immunoblot was performed on control, early AD, and AD patient brain tissues using the mouse version of this antibody to determine the link between the complement system and Alzheimer’s disease (Wu et al, 2019; pmid:31433986). To study the deposition of the complement protein fragment C3b and its breakdown products on CD20-positive cells, flow cytometry was performed on Raji or ARH-77 cells using the mouse version of this antibody. Furthermore, a radioimmunoassay was performed on Raji or ARH-77 cells using this antibody. Finally, Raji or ARH-77 cells were stained for immunofluorescence using this antibody (Kennedy et al, 2003; pmid:12393727). To study how Rituximab activates complement and promotes covalent deposition of C3 fragments (C3b/iC3b) on cells, flow cytometry was performed on raji cells in presence of the mouse version of this antibody. Furthermore, raji cells were stained for immunofluorescence using this antibody (Beum et al, 2006; pmid:17067631). To study the alternative pathway (AP) of the complement system, flow cytometry was performed on AP-mediated C3b-opsonizated zymosan using the mouse version of this antibody. Furthermore, immunofluorescence was performed on AP-mediated C3b-opsonizated zymosan using this antibody (DiLillo et al, 2006; pmid:15961157).
Antibody first published in:
Wu et al. Preparation of monoclonal antibodies to C3b by immunization with C3b(i)-sepharose J Immunol Methods. 1989 Jun 21;120(2):241-9. PMID:2786912
Note on publication:
Four monoclonal antibodies were prepared and characterized to human C3b of high specificity and affinity.