United Kingdom (UK)
Recombinant monoclonal antibody to CCR7. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from The hybridoma 4B12.
UniProt Accession Number of Target Protein: P47774
Alternative Name(s) of Target: EBI1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus-induced G-protein coupled receptor 1; MIP-3 beta receptor; CD197; C-C CKR-7; CC-CKR-7; C-C chemokine receptor type 7
Immunogen: This antibody was raised by immunizing rats with mouse CCR7 antigen.
Specificity: This antibody is specific to the mouse CCR7 protein, which was confirmed by immunoprecipitation (Ritter et al, 2004).
Application Notes: This antibody was used for flow cytometry and the staining quality can be improved by incubation at 37°C (Britschgi et al, 2008). This antibody is widely used to monitor the activation of dendritic cells in mice (Ohl et al, 2004; Ritter et al, 2004; Zhao et al, 2019). It was also utilized in immunoprecipitation of the CCR7 receptor protein (Ritter et al, 2004). The antibody does not interfere with ligands binding to the CCR7 receptor, which was confirmed by a transmigration assay (Ritter et al, 2004). It does not interfere with T-cell migration (Ritter et al, 2004). The antibody also is not agonistic as it does not activate the receptor (Ritter et al, 2004).
Antibody first published in:
Ritter et al. Analysis of the CCR7 expression on murine bone marrowderived and spleen dendritic cells. J Leukoc Biol. 2004 Aug;76(2):472-6. Epub 2004 Jun 14. PMID:15197229
Note on publication:
The article describes the analysis of CCR7 expression during the maturation of bonemarrow-dendritic cells
Immunofluorescence staining of fixed RAW264.7 cells with anti-CCR7 antibody 4B12 (Ab01590) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 4B12 (Ab01590-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01590-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.