United Kingdom (UK)
Recombinant monoclonal antibody to CD14. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MiL2.
UniProt Accession Number of Target Protein: P08571
Alternative Name(s) of Target: Monocyte antigen; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
Immunogen: The original antibody was raised by immunizing a mouse with porcine CD14.
Specificity: This antibody is specific for porcine CD14.
Application Notes: Western blot was performed using this antibody on proteins (US20170107294). This antibody was used for flow cytometery on fresh human or porcine whole blood (US20170107294) (Thorgersen et al, 2009; pmid:19047409). The biological effect of this antibody on the inflammatory response of piglets was tested (US20170107294). ELISA was performed on C1q and human FcγRs using this antibody (US20170107294). Immunofluorescence was performed on both PBMC and whole blood samples using this antibody (Zelnickova et al, 2007; pmid:17720184). Immunofluorescent staining was performed on cardiac and diaphragmatic lung lobes using this antibody (Gucht et al, 2005; pmid:15802956).
Antibody first published in:
Gucht et al. Porcine reproductive and respiratory syndrome virus infection increases CD14 expression and lipopolysaccharide-binding protein in the lungs of pigs. Viral Immunol. 2005;18(1):116-26. PMID:15802956
Note on publication:
The levels of CD14 expression and LPS-binding protein (LBP) in the lungs of pigs throughout a PRRSV infection were quantified.
Immunofluorescence staining of U937 cells with anti-CD14 antibody MiL2 (Ab02474). Immunofluorescence analysis of paraformaldehyde-fixed U937 cells on Shi-fix™ coverslips stained with the mouse IgG2b version of MiL2 (Ab02474-3.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02474-3.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Western blot using anti-CD14 antibody MiL2 (Ab02474). U937 cells lysate (35µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the mouse version of MiL2 (Ab02474-3.0) at 1µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.