United Kingdom (UK)
Recombinant monoclonal antibody to CD16 (FCgRIII). Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma B88-9.
UniProt Accession Number of Target Protein: Q9ULV2
Alternative Name(s) of Target: Low Affinity IgR; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; FCGR3A; CD16A; FCG3; FCGR3; IGFR3; Fc-gamma receptor IIIb
Immunogen: This antibody was raised against FCgRIII expressing cells.
Specificity: B88-9 binds to human CD16 at an epitope comprised of LEKDSVTK (aa 21-27), (SWKNTALH (aa 113-120), FHHNS (aa 134-138) and VGSKN (aa 158-162) (Feng et al, 2004). hCD16 is recognized by B88-9 when both bound and unbound to hIgG1 – B88-9-bound CD16 cannot be bound by hIgG1.
Application Notes: This antibody binds to CD16, a low affinity Fc receptor on the surface of natural killer cells, neutrophils, monocytes and macrophages.
Antibody first published in:
Tamm A, Schmidt RE. The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81 PMID:8759741
Note on publication:
Describes the characterization of this antibody.
Flow-cytometry using the anti-CD16 (FCgRIII) antibody B88-9 (Ab00218). paraformaldehyde fixed human peripheral blood leukocytes were stained with an isotype control (Ab00102-23.0, black line) or the rabbit-chimeric version of B88-9 (Ab00218-23.0, blue line) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488® conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.