United Kingdom (UK)
Recombinant monoclonal antibody to CD18. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma YFC51.1.
UniProt Accession Number of Target Protein: P05107
Alternative Name(s) of Target: ITGB2; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; β2-subunit of leukocyte integrins
Immunogen: The original antibody was generated by immunizing DA rats with human neutrophils.
Specificity: This antibody binds human CD18 and recognizes amino acid residues between 122 and 344, more particularly amino acids from two regions 123–163 and 302–344 of the human CD18 molecule (PMID: 10779511). Binding of CD18 and CD11 results in the formation of Lymphocyte Functions Associated Antigen 1 (LFA-1), a protein found on B cells, all T cells, macrophages, neutrophils and NK cells. LFA-1 is involved in adhesion and binding to antigen presenting cells through interactions with the surface protein ICAM-1.
Application Notes: The original rat IgG2b version of this antibody was successful in blocking CD18. The human IgG1 version of this antibody was able to block CD18 but unable to bind human Clq and did not mediate antibody-dependent cellular cytotoxicity (PMID: 7688398). This antibody was used as one of the antibodies to study the effect of anti-LFA-1 panel of abs on T cell adhesion to ICAM-1 and ICAM-3 using ELISA (PMID: 7518468).
Antibody first published in: Cobbold S, Hale G, Waldmann H. Non-lineage, LFA-I family, and leukocyte common antigens: new and previously defined clusters. Leukocyte Typing Ill. White Cell Differentiation Antigens. A. J. McMichael, editor. Oxford University Press, Oxford. 784-803. ISBN 10: 0192615521 / ISBN 13: 9780192615527 PMID: