United Kingdom (UK)
Recombinant monoclonal antibody to CD20. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CAT-13.6E12.
UniProt Accession Number of Target Protein: P11836
Alternative Name(s) of Target: Leu16; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CAT 13.6E12
Immunogen: This antibody was raised by immunising mice against CD20.
Specificity: This antibody recognises human CD20, which is a B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes.
Application Notes: CAT 13.6E12, as an anti-CD20 antibody, can be used to detect normal and neoplastic B cells in tissue sections as well as to identify CD20 via flow cytometry. This antibody was also recommended for treating primary intraocular lymphoma (WO2010076400A1).
Antibody first published in:
Fisson et al. Use of an anti-cd20 antibody for treating primary intraocular lymphoma U.S. Patent Application 13/143,056. PMID:
Note on publication:
This patent describes CAT 13.6E12 antibody.
Immunofluorescence staining of fixed Daudi cells with anti-CD20 antibody CAT-13.6E12 (Ab01503) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of CAT-13.6E12 (Ab01503-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01503-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CD20 antibody CAT-13.6E12 (Ab01503). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of CAT-13.6E12 (Ab01503-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.