United Kingdom (UK)
Recombinant monoclonal antibody to CD34. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2E10.
UniProt Accession Number of Target Protein: P28906
Alternative Name(s) of Target: Hematopoietic progenitor antigen;Hematopoietic progenitor cell antigen CD34; Q3C1E9
Immunogen: This antibody was raised by immunising BALB/c mice with KG-1a cells, a human acute myeloid leukaemia cell line.
Specificity: This antibody is specific for human CD34, as confirmed by western blot analysis, and recognises a class III epitope.
Application Notes: This antibody effectively binds to CD34-expressing KG-1a cells in FC analysis, and has been shown to react specifically with CHO-CD34 cells, but not CHO cells (Qian et al, 2008). This antibody has also been shown to bind CD34 in western blot analysis (Qian et al, 2008).
Antibody first published in:
Qian et al. Development of new versions of anti-human CD34 monoclonal antibodies with potentially reduced immunogenicity Biochemical and Biophysical Research Communications; Volume 367, Issue 2, 7 March 2008, Pages 497-502 PMID:
Note on publication:
Describes the original generation of this antibody, and its characterisation and use in FC, WB and ELISA analysis.