United Kingdom (UK)
Recombinant monoclonal antibody to CD48. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma IIID3.
UniProt Accession Number of Target Protein: P09326
Alternative Name(s) of Target: BLAST-1; CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2; SLAMF2; 111D3; Signaling lymphocytic activation molecule 2; TCT.1
Immunogen: This antibody was raised by immunizing mice against CD48.
Specificity: The antibody IIID3 recognizes and is directed against CD48.
Application Notes: The clone IIID3 is recommended for the identification and analysis of CD48.
Antibody first published in: PMID:
Flow-cytometry using the anti-CD48 antibody IIID3 (Ab01528). Paraformaldehyde fixed human peripheral blood leukocytes were stained with an isotype control (Ab00102-23.0, black line) or the rabbit-chimeric version of IIID3 (Ab01528-23.0, blue line) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488® conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.
Immunofluorescence staining of Daudi cells with anti-CD48 (Ab01528) IIID3 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric rabbit IgG version of IIID3 (Ab01528-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01528-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (Ab00102-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.