United Kingdom (UK)
Recombinant monoclonal antibody to CD56. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma NCAM12.19.
UniProt Accession Number of Target Protein: P13591
Alternative Name(s) of Target: Ncam; NCAM1; HCD56; NKH-1; Neural Cell Adhesion Molecule; Leu-19; NKH1
Immunogen: This antibody was raised against human NCAM/CD56
Specificity: This antibody is specific for human CD56, which is expressed on NK cells, as well as in the brain and at neuromuscular junctions.
Antibody first published in: PMID:
Binding curve of anti-CD56 antibody NCAM12.19 (Ab01426-23.0) to recombinant human NCAM1 Fc-Fusion Protein. ELISA Plate coated with recombinant human NCAM1 Fc-Fusion Protein (Pr00241-10.9; Absolute Antibody) at a concentration of 5 µg/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using Ab01426-23.0. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.
Western Blot using anti-CD56 antibody NCAM12.19 (Ab01426). human brain cerebellum(A) and cerebral cortex(B) tissue lysate (35µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of NCAM12.19 (Ab01426-1.1) at 0.03 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence staining of Kelly cells using anti-CD56 (Ab01426) NCAM12.19 Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric mouse IgG version of NCAM12.19 (Ab01426-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01215-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.
Western Blot using anti-CD56 antibody NCAM12.19 (Ab01426). human brain cerebellum(A) and cerebral cortex(B) tissue lysate (35µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of NCAM12.19 (Ab01426-1.1) at 0.03 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.