United Kingdom (UK)
Recombinant monoclonal antibody to CD79a. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma JCB117.
UniProt Accession Number of Target Protein: P11912
Alternative Name(s) of Target: MB-1; B-cell antigen receptor complex-associated protein alpha chain; Ig-alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein; MB-1 membrane glycoprotein
Immunogen: This antibody was raised by immunising mice with a recombinanat mb-1 protein followed by the preparation of a murine hybridoma.
Specificity: This antibody recognises CD79a which is involved in initiation of the signal transduction cascade activated by binding of antigen to the B-cell receptor. CD79a is used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissue sections.
Application Notes: This antibody reacts very well with B-cells during immunohistochemical labelling of paraffin-embedded tissue samples. It detected most B-cell neoplasms tagging B-cells at various stages of maturation (Mason et al., 1995). Consequently, it may be used as a reliable marker in the detection and investigation of different B-cell conditions. For instance, it labels precursor B-cell acute lymphoblastic leukemia samples what makes it the most efficient marker of this disorder in paraffin-embedded tissue (Mason et al., 1995). Furthermore, this antibody may be useful in staining and identification of plasma cell myeloma and acute promyelocytic leukemia. For example, JCB117 antibody was utilised in the characterisation of multiple myeloma and it showed very strong immunostaining of the neoplastic cells (Requena et al., 2003).
Antibody first published in:
Mason et al. CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood. 1995 Aug 15;86(4):1453-9. PMID:7632952
Note on publication:
This article describes generation of JCB117 antibody and its application in immunohistochemistry.
Immunofluorescence staining of Daudi cells using anti-CD79a (Ab01332) JCB117 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric mouse IgG version of JCB117 (Ab01332`-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01215-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.
Western Blot using anti-CD79a antibody JCB117 (Ab01332). Daudi cell lysate(A) (0.01 µg/ml) and human tonsil(B) tissue lysate (0.03 µg/ml) (35µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of JCB117 (Ab01332-1.1) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow cytometry using the Anti-CD79a antibody JCB117 (Ab01332). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of JCB117 (Ab01332-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.