United Kingdom (UK)
Recombinant monoclonal antibody to CD79b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AT107-2.
Alternative Name(s) of Target: B-cell antigen receptor complex-associated protein beta chain; CD79B; B-cell-specific glycoprotein B29; Ig-beta; Immunoglobulin-associated B29 protein
Immunogen: The rat version of this antibody was raised by immunizing a mouse with the intracellular domain of CD79B.
Specificity: This antibody specific for the intracellular domain of human CD79b. CD79b is required in tandem with CD79A for initiation of the signal transduction cascade induced by the B-cell antigen receptor complex which leads to internalization of the complex, trafficking to late endosomes and antigen presentation.
Application Notes: To study chronic lymphocytic leukemia, immunohistochemistry was performed on tissues from patients with CLL or RT using the rat version of this antibody (Allan et al, 2019). Tissue from non-Hodgkin lymphoma and chronic lymphocytic leukemia patients was stained for immunohistochemistry using the rat version of this antibody to create an antibody–drug conjugate consisting of a humanized anti-CD79b antibody that is conjugated to monomethylauristatin E. This construct is supposed to help the patients with these forms of cancer (Dornan et al, 2009; pmid:19633198). To investigate anti CD79b clone DCDS0780A as a possible therapeutic agent against B-NHL, immunohistochemistry was performed on tissues from B-NHL patients that had relapsed after or failed to respond to at least one prior treatment regimen using the rat version of this antibody (Herrera et al, 2022; pmid:34980599). To determine the expression of CD79b in tumor cells immunohistochemistry was performed on tissues from R/R DLBCL patients using the rat version of this antibody (Sehn et al, 2019; pmid:31693429). To determine the lymphoma isotype of dogs, flow cytometry was performed on lymph node cells using the rat version of this antibody (Thalheim et al, 2013; pmid:24112291). To understand immune activity in koalas, flow cytometry was performed on peripheral blood leukocytes of koalas using the rat version of this antibody (Lau et al, 2012; pmid:22551982). To characterize a CD4+ CD25 high FOXP3 high regulatory T-cell population in dogs, flow cytometry was used on PMBCs using the rat version of this antibody (Pinheiro et al, 2011; pmid:20880379).
Antibody first published in:
Dornan et al. Therapeutic potential of an anti-CD79b antibody-drug conjugate, anti-CD79b-vc-MMAE, for the treatment of non-Hodgkin lymphoma Blood. 2009 Sep 24;114(13):2721-9. PMID:19633198
Note on publication:
An antibody-drug conjugate (ADC) consisting of a humanized anti-CD79b antibody that is conjugated to monomethylauristatin E was created.