Anti-CD79b [HM79-16]

Name: Anti-CD79b [HM79-16]
SKU: Ab01573
Availability: InStock

Description:

Recombinant monoclonal antibody to CD79b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HM79-16.

Clone Name:

HM79-16

Published Application(s):

IP, FC, IF, IHC

Published Species Reactivity:

Mouse

Products based on this clone:

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Ab01573-22.0 Anti-CD79b [HM79-16]
Mouse
Hamster (Armenian) IgG
Purified
In Stock

Ab01573-3.0 Anti-CD79b [HM79-16]
Mouse
Mouse IgG2b
Purified
Ships in 4-5 weeks

Ab01573-3.3 Anti-CD79b [HM79-16]
Mouse
Mouse IgG2b
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01573-1.32 Anti-CD79b [HM79-16]
Mouse
Mouse IgG1-D265A
Fc Silenced
Purified
Ships in 4-5 weeks

Ab01573-23.0 Anti-CD79b [HM79-16]
Mouse
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: P15530

Alternative Name(s) of Target: Ig-beta; AGM6; B29; IGB; CD79b molecule;
Immunogen: HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).

Specificity: Reacts with an extracellular epitope of CD79b.

Application Notes: A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008).

Antibody first published in: Mariko Koyama et al. CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated µ heavy chain International Immunology, Vol. 9, No. 11, pp. 1767–1772 1997 PMID:9418137
Note on publication: Describes the expression of the CD79b and the use of new monoclonal antibodes to detect CD79b on pro-B cell surfaces.

IF staining of mouse splenocytes using Ab01573-23.0. Click on image for more details.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD79b antibody HM79-16 (Ab01573) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version HM79-16 (Ab01573-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01573-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot on mouse spleen tissue lysate. Using Ab01573-23.0. Click on image for more details.

Western Blot using anti-CD79b antibody HM79-16 (Ab01573) Mouse spleen tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HM79-16 (Ab01573-23.0) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

IF staining of mouse splenocytes using Ab01573-23.0. Click on image for more details.

Flow cytometry analysis of mouse splenocytes using Ab01573-23.0. Click on image for more details.

Flow cytometry using the Anti-CD79b antibody HM79-16 (Ab01573). Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of HM79-16 (Ab01573-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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