United Kingdom (UK)
Recombinant monoclonal antibody to CD89. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 14A8 (14.1).
UniProt Accession Number of Target Protein: P24071
Alternative Name(s) of Target: Immunoglobulin alpha Fc receptor; FCAR; IgA Fc receptor; FcαR
Immunogen: The original antibody was generated by immunizing HCO12 trangene containing mice with recombinant soluble CD89 antigen.
Specificity: This antibody binds CD89 in the extracellular domain 2, outside the IgA-binding site.
Application Notes: This antibody binds human CD89 with a dissociation constant of Kd= 6.8 X10-10M.The binding of this antibody to human CD89 was confirmed with ELISA. This antibody was also capable of recognizing human CD89 expressing cells like human PMNL cells by flow cytometry. This antibody is not capable of blocking binding of IgA to CD89. This antibody can used to immunoprecipitate soluble CD89 (US7192582). A bispecific antibody construct incorporating the variable regions of the anti-gp41 antibody F240 and the anti-CD89 (immunoglobulin A [IgA] receptor) antibody 14A8 was reported to promote destruction of HIV type 1 (HIV-1) by neutrophils (PMID: 18272577). A Fab-like bispecific antibody molecule or Bi-Fab antibody generated from F240scFv and 14A8scFv showed significant antiboy dependent cell-mediated viral inhibition (ADCVI) activity for multiple clade B and clade C isolates by arming the neutrophils to inhibit HIV infection (PMID: 27419146).
Antibody first published in:
Fishwild et al. High-avidity human IgG kappa monoclonal antibodies from a novel strain of minilocus transgenic mice. Nat Biotechnol. (1996); 14(7):845-51. PMID:9631008
Note on publication:
Describes the generation of this antibody.
Flow cytometry using the Anti-CD89 antibody 14A8 (14.1) (Ab02426). Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 14A8 (14.1) (Ab02426-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.