United Kingdom (UK)
Recombinant monoclonal antibody to CDX2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 12B6.
UniProt Accession Number of Target Protein: Q99626
Alternative Name(s) of Target: Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2; rabbit mono-ERP2764Y; TA347
Immunogen: The original antibody was generated by immunizing a mouse with the CDX2-KLH protein.
Specificity: The antibody recognises human CDX2. The CDX2 protein is a homeobox transcription factor expressed in the nuclei of intestinal epithelial cells, playing an essential role in the development and function of the digestive system.
Application Notes: The antibody was used for immunohistochemistry on stomach adenocarcinoma, showing accurate staining location, clear staining, no non-specific staining, and clean background. Further, immunohistochemistry staining on stomach adenocarcinoma from 12B6 antibody and the commercial antibody, rabbit mono-ERP2764Y, were compared. Results showed the staining intensity of 12B6 antibody was significantly higher than those of ERP2764Y, showing 12B6 antibody is more sensitive. Moreover, the antibody and the commercial antibody TA347 were used for immunohistochemistry on appendix tissue. Results showed that the antibody (12B6) and the commercially available antibody (TA347) were simultaneously detected on the normal tissue chip, and the negative and positive detection results were consistent, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody. The antibody detected human CDX2 by western blot analysis (CN113265000).
Antibody first published in: PMID:
Flow cytometry using anti-CDX2 antibody 12B6 (Ab03239). Paraformaldehyde fixed Caco-2 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 12B6 (Ab03239-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.
Western blot using anti-CDX2 antibody 12B6 (Ab03239). Caco-2 (A) (0.003µg/ml) cells lysate and human colorectal carcinoma (B) (0.003µg/ml) tissue lysate (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 12B6 (Ab03239-23.0) at the mentioned respective concentration before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.
Immunofluorescence staining of Caco-2 cells with anti-CDX2 antibody 12B6 (Ab03239). Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 12B6 (Ab03239-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03239-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.