United Kingdom (UK)
Recombinant monoclonal antibody to CEACAM1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma C5-1X/8.
UniProt Accession Number of Target Protein: P13688
Alternative Name(s) of Target: CD66a; Carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; BGPI
Immunogen: This antibody was raised by immunising mice with recombinant human soluble CEACAM-1-Fc, produced in HEK293 cells.
Specificity: This antibody is specific for human CEACAM1, and targets the targets the A1-B-domain.
Application Notes: This antibody has been used in ELISA analysis to detect and quantify soluble CEACAM1 in human bronchoalveolar lavage fluid samples (Klaile et al, 2013). This antibody has also been used in immunohistochemical staining of paraffin-embedded human brain tissue sections, revealing CEACAM1-positive B cells in brain infiltrates (Rovituso et al, 2016). Additionally, this antibody has been used in Western blot analysis of CEACAM1 expression in human intestinal epithelial cell lysates (Klaile et al, 2017). This antibody does not inhibit B cell aggregation in vitro (Rovituso et al, 2016).
Antibody first published in:
Klaile et al. Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons. Respir Res. 2013 Aug 14;14:85 PMID:23941132
Note on publication:
Describes the original use of this antibody, in a CEACAM1-specific sandwich ELISA.
Immunofluorescence staining of HepG2 cells using anti-CEACAM1 antibody (Ab01368) C5-1X/8 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of C5-1X/8 (Ab01368-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01368-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-CEACAM1 antibody C5-1X/8 (Ab01368). HepG2 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of C5-1X/8 (Ab01368-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-CEACAM1 antibody C5-1X/8 (Ab01368) Human colon (A) and human liver (B) tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of C5-1X/8 (Ab01368-23.0) at 0.3 µg/ml and 0.03 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence staining of HepG2 cells using anti-CEACAM1 (Ab01368) C5-1X/8 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric mouse IgG version of C5-1X/8 (Ab01368-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01215-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CEACAM1 antibody C5-1X/8 (Ab01368). Paraformaldehyde fixed HepG2 cells were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of C5-1X/8 (Ab01368-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.