United Kingdom (UK)
Recombinant monoclonal antibody to CHD4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody RAB-C212.
UniProt Accession Number of Target Protein: Q14839
Alternative Name(s) of Target: CHD-4; Mi2; Mi-2; chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; chromatin ATP-dependent helicase domain; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta; CHD4-RAB-C212; CHD4-A001-1313
Immunogen: The original antibodywas isoltated from an optimised automated phage display library by panning against specific antigens.
Specificity: This antibody binds human chromodomain-helicase-DNA-binding protein 4 (CHD4). It is a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in the gene that encodes this protein are associated with serous endometrial tumors.
Application Notes: The binding specificity of this antibody to human CHD4 was confirmed with ELISA. This antibody was also used in the immunofluorescense staining of 6 human cell lines (PMID: 26290498).
Antibody first published in:
Hornsby et al. A High Through-put Platform for Recombinant Antibodies to Folded Proteins Mol Cell Proteomics. 2015 Oct; 14(10): 2833–2847. PMID:26290498
Note on publication:
Describes the generation of optimized automated phage display and antigen expression pipeline to generate recombinant antibodies against transcription factors (TFs) and epigenetic antigens.