United Kingdom (UK)
Recombinant monoclonal antibody to CPS-500. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 18.44.
Alternative Name(s) of Target: β-mannosylated glycolipid, CPS-500 antigen
Immunogen: The original antibody was generated by immunizing mice with whole cells of Cryptosporidium parvum.
Specificity: This antibody binds the CPS-500 antigen of sporozoites and merozoites of Cryptosporidium parvum. Cryptosporidium parvum is a coccidian parasite that infects intestinal epithelium and causes diarrheal disease in humans and other agriculturally important food animals for which specific therapy and immunoprophylaxis are unavailable. Cryptosporidiosis is self-limiting in hosts with normal immune systems; however, in neonates, the elderly, and hosts having congenital or acquired immunodeficiency diseases or chemotherapy-induced immunosuppression, cryptosporidial enterocolitis may become chronic and have severe consequences.
Application Notes: This antibody reacts with both sporozoites and merozoites of C. parvum in an indirect immunofluorescence assay. This antibody is capable of neutralizing sporozoites and merozoites in a time dependent manner (PMID: 2193057; 2473130). This antibody can be used for the identification of sporozoites, merozoites and CPS-500 antigen of C. parvum using western blot. The identification of sporozoite-neutralizing antibody was done using ELISA (PMID: 20086143). This antibody in combination with PLA2 or LL37 was also used in the generation of a biocide fusion proteins to kill C. parvum sporozoites in vitro and in vivo (PMID: 20086143).
Antibody first published in:
Bjorneby et al. Cryptosporidium parvum merozoites share neutralization-sensitive epitopes with sporozoites. J Immunol. 1990 Jul 1;145(1):298-304. PMID:2193057
Note on publication:
Describes the use of antibodies to determine common epitopes shared between merozoites and sporozoites of C. parvum.