United Kingdom (UK)
Recombinant monoclonal antibody to CRP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma P20.
UniProt Accession Number of Target Protein: P02741
Alternative Name(s) of Target: C reactive protein; PTX1; C-reactive protein
Immunogen: The original antibody was generated by immunizing BALB/c mice with C-reactive protein extracted from human plasma.
Specificity: This antibody reacts with human C reactive protein, which is a pentameric protein found in blood plasma. It is an acute phase reactive protein of hepatic origin. It is an important defense molecule for the body and its plasma concentration increases sharply during tissue damage, inflammation and bacterial infection. It promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Application Notes: Indirect ELISA was used for the primary screening of the hybridomas showing selective binding for human CRP. Horseradish peroxide labelled scFv P20 was used for the identification of natural CRP extracted from denatured and non-denatured blood in a western blot assay. The combination of P02 and P20 antibody was also used in the double antibody sandwich detection system consisting of P20 as the coating antibody and P02 as the labeled antibody to be used in the colloidal gold detection platform for the detection of natural CRP. These antibodies were also used in the preparation of human C-reactive protein colloidal gold test card (CN106596976).
Antibody first published in: PMID:
Flow-cytometry using the anti-FLT3 antibody NC7 (Ab03014). HepG2 cells were stained with an isotype control (Ab00102-23.0, black line) or the rabbit-chimeric version of NC7 (Ab03014-23.0, blue line) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488® conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.
Immunofluorescence staining of HepG2 cells with anti-CRP (Ab03014) P20 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of P20 (Ab03014-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab03014-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (Ab00102-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-FLT3 antibody NC7 (Ab03014). HepG2 cells were stained with an isotype control (Ab00102-23.0, black line) or the rabbit-chimeric version of NC7 (Ab03014-23.0, blue line) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488® conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.