United Kingdom (UK)
Recombinant monoclonal antibody to Cortisol. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5A4.
Alternative Name(s) of Target: Hydrocortisone; ChEBI: 17650
Immunogen: The original antibody was generated by immunizing mice with cortisol-7-CM, followed by cortisol-3-CMO. Both forms of cortisol were bound to BSA.
Specificity: This antibody binds cortisol. It has also shown cross-reactivity to dexamethasone and prednisolone. This is due to a hydroxyl group bound to C11. Cortisol is a steroid hormone, in the glucocorticoid class of hormones. It is produced in many animals, mainly by the zona fasciculata of the adrenal cortex in the adrenal gland. It is produced in other tissues in lower quantities. It is released with a diurnal cycle and its release is increased in response to stress and low blood-glucose concentration. It functions to increase blood sugar through gluconeogenesis, to suppress the immune system, and to aid in the metabolism of fat, protein, and carbohydrates. It also decreases bone formation. This antibody has not shown any affinity for cortisol-21-hemisuccinate-BSA.
Application Notes: The specificity and affinity of this mouse version of this antibody was determined using equilibrium dialysis in dialysis cells fitted with a cellulose membrane of 3500 MW cutoff (Calvez et al, 1995; pmid:7898495). Further a radioimmunoassay was performed on I125-cortisol using the scFv version of this antibody (Calvez et al, 1995; pmid:7898495). The specificity of this antibody towards cortisol-3-CMO-BSA, cortisol, prednisolone and dexamethasone was also tested using equilibrium dialysis. ELISA was used to show that this clone was able to bind steroids conjugated to BSA through nonspecific hydrophobic interactions (PMID: 9820517).
Antibody first published in:
Calvez et al. Paratope characterization by structural modelling of two anti-cortisol single-chain variable fragments produced in E. coli. Mol Immunol. 1995 Feb;32(3):185-98. PMID:7898495
Note on publication:
Two monoclonal antibodies (mAbs), 5A4 and 6D6, directed against cortisol, have been obtained; 6D6 is used in an assay kit for cortisol. The antibodies also recognize other, structurally related steroids present in the sample assayed.