United Kingdom (UK)
Recombinant monoclonal antibody to Cotinine. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CT#45.
Alternative Name(s) of Target: 486-56-6; ChEBI:68641; (S)-Cotinine; Cotinina; Cotininum
Immunogen: The original antibody was generated by immunizing BALB/c mice with a cotinine–albumin conjugate.
Specificity: This antibody binds cotinine. Cotinine is an alkaloid found in tobacco and is also the predominant metabolite of nicotine. It is used as a biomarker for exposure to tobacco smoke.
Application Notes: This antibody was used in the development of an ELISA for determining urinary cotinine to monitor tobacco smoke exposure. This ELISA produced dose–response curves for cotinine ranging from 0.40–100 ng per assay and was able to discriminate nicotine, cotinine N-oxide, cotinine N-glucuronide (cross-reactivity was each <0.5%, taking cotinine as 100%), (3′R, 5′S)-3′-hydroxycotinine O-glucuronide (0.6%) and (R, S)-norcotinine (1.5%). Another major nicotine metabolite, (3′R, 5′S)-3′-hydroxycotinine, showed 8.4% cross-reactivity with this antibody (Kobayashi, 2011)
Antibody first published in:
Kobayashi et al. A monoclonal antibody-based enzyme-linked immunosorbent assay for human urinary cotinine to monitor tobacco smoke exposure. Analytical Methods 2011, Issue 9. PMID:
Note on publication:
Describes the use of this antibody to develop an ELISA to detect urinary cotinine.