United Kingdom (UK)
Recombinant monoclonal antibody to Cytochrome b-245 light chain. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma NS5.
UniProt Accession Number of Target Protein: P13498
Alternative Name(s) of Target: CYBA; flavocytochrome b; Cyt b; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox
Specificity: The antibody recognises Cytochrome b-245 light chain (Epitope LLEYPRG+VKLFGP).
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected p22phox by western blot analysis. Further, the antibody immunoprecipitated Cyt b. The antibody showed moderate levels of inhibition of superoxide production by the NADPH oxidase complex (Taylor et al., 2004; PMID: 15585859).
Antibody first published in:
Taylor et al. Site-specific inhibitors of NADPH oxidase activity and structural probes of flavocytochrome b: characterization of six monoclonal antibodies to the p22phox subunit J Immunol. 2004 Dec 15;173(12):7349-57. PMID:15585859
Note on publication:
The original paper describes the generation and characterization of the antibody.