United Kingdom (UK)
Recombinant monoclonal antibody to DMC1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2H12/4.
UniProt Accession Number of Target Protein: Q14565
Alternative Name(s) of Target: Meiotic recombination protein DMC1/LIM15 homolog; DMC1H; LIM15; MGC150473; MGC150472; HsLim15; DMC1 homologue; disrupted meiotic cDNA1; hDMC1
Immunogen: This antibody was raised against human DMC1 protein (expressed originally as a 6xHis fusion in E. coli).
Specificity: This antibody is specific for DMC1 and does not cross-react with the related protein Rad51. DMC1 is a RecA/RAD51 homologue which is specific for meiosis and critical for meiotic recombination in Eukaryota.
Application Notes: IF: 2H12/4 anti-DMC1 antibody was used to visualise DMC1 on chromatin in I-L2-NS endopolyploid tumour cells in experiments investigating the role of another protein - REC8 in chromosome segregation after radiation (Erenpreisa et al., 2009). This antibody was also utilised to stain paraffin-embedded cervical cancer tissue samples against DMC1 (Ianzini et al., 2009). IF: Hopkins et al. (2014) successfully used this antibody to stain chromatin spreads from purified testicular germ cells of Stag3+/− and Stag3−/− mice for DMC1 in the studies on the role of Stag3. IF: Another group also used this antibody for the immunofluorescence staining of spermatocyte chromosome spreads against DMC1 in a study analysing proteins involved in telomeres' attachment to the nuclear envelope (Wang et al., 2019).
Antibody first published in:
Erenpreisa et al. The role of meiotic cohesin REC8 in chromosome segregation in γ irradiation-induced endopolyploid tumour cells Exp Cell Res. 2009 Sep 10;315(15):2593-603. doi: 10.1016/j.yexcr.2009.05.011. Epub 2009 May 20. PMID:19463812
Note on publication:
This article describes the earliest use of this anti-DMC1 antibody. It was utilised to visualise DMC1 protein on chromosomes of endopolyploid tumour cells in experiments investigating the role of another protein - REC8 in chromosome segregation after radiation.
Immunofluorescence staining of fixed HeLa cells with anti-DMC1 antibody 2H12/4 (Ab01311) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 2H12/4 (Ab01311-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01311-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.