United Kingdom (UK)
Recombinant monoclonal antibody to DNA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4B1.
Alternative Name(s) of Target: ssDNA; actin; tubulin; cardiolipin; laminin.
Immunogen: The original antibody was derived from a 12 month old (NZB × NZW)F1 lupus mouse
Specificity: This antibody is a polyspecific antibody that has binding specificity for ssDNA, actin, tubulin, cardiolipin and laminin.
Application Notes: This antibody is capable of binding ssDNA, actin, tubulin, cardiolipin and to laminin in a solid phase ELISA. Indirect immunofluorescence labeling of HEp-2 cells gave a cytoplasmic staining pattern similar to that obtained with anti-cytoskeleton antibodies. Western blot analysis demonstrated that mAb 4B1 bore idiotype D23, previously shown to be characteristic of natural antibodies derived from normal mice. This antibody was able to form glomerular immune deposits (Gilbert et al., 1995; PMID: 7540257).
Antibody first published in:
Gilbert et al. An idiotype D23-bearing polyspecific, murine anti-DNA monoclonal antibody forms glomerular immune deposits. Pathogenic role of natural autoantibodies? Mol Immunol. 1995 May;32(7):477-86. PMID:7540257
Note on publication:
Describes the generation and characterization of a polyspecific anti-DNA antibody that forms glomerular immune deposits.