United Kingdom (UK)
Recombinant monoclonal antibody to DNMT3L. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N117/9.5.
UniProt Accession Number of Target Protein: Q9UJW3
Alternative Name(s) of Target: N117/9.5R; DNA (cytosine-5)-methyltransferase 3-like
Immunogen: This antibody was raised by immunising BALB/c mice with the DNMT3L antigen.
Specificity: This antibody is specific of the DNMT3L antigen. This protein is a catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity).
Application Notes: This antibody is recommended for the detection and analysis of DNMT3L by western blot, immunoflurescence and immunohistochemistry. For instance, the mouse version of this antibody was used to detect DNMT3L by western blot in COS cells transiently transfected with untagged Kv2.1 and Flag-tagged DNMT3L. It was also used to detect DNMT3L by immunofluorescence in COS cells expressing Myc-tagged human DNMT3L.
Antibody first published in:
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication:
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.