United Kingdom (UK)
Recombinant monoclonal antibody to DUX4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma P2B1.
UniProt Accession Number of Target Protein: Q9UBX2
Alternative Name(s) of Target: Double homeobox protein 4; Double homeobox protein 10; DUX10; DUX4L1; P2B1-B3
Immunogen: This antibody was raised by immunising mice with C-terminal fragment of human of DUX4 (aa 349-424) with glutathione-s-transferase (gst) tag.
Specificity: This antibody recognises C-terminal fragment of human DUX4-fl, aa 349-424. It does not cross-react with DUX4c. DUX4 is a protein thought to be engaged in facioscapulohumeral dystrophy (FSHD), one of the most common muscular dystrophies.
Application Notes: P2B1 antibody can be used to assess the protein expression of DUX4 in muscle cells affected by facioscapulohumeral dystrophy (FSHD). For instance, this antibody was used to stain C2C12 myoblasts transfected with human DUX4 (Geng et al., 2011).
Antibody first published in:
Geng et al. Immunodetection of human double homeobox 4. Hybridoma (Larchmt). 2011 Apr;30(2):125-30. doi: 10.1089/hyb.2010.0094. PMID:21529284
Note on publication:
This article describes the generation and characterisation of the P2B1 antibody.