United Kingdom (UK)
Recombinant monoclonal antibody to Diethoxyphosphorothioate. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 12C2.
Alternative Name(s) of Target: O,O-diethyl organophosphorus pesticides; DOPs; OPs
Immunogen: The antibody was generated by immunizing BABL/c mice with the diethoxyphosphorothioate derivated hapten, followed by antibody library construction and phage display-based selection.
Specificity: The antibody is specific for a broad set of O,O-diethyl organophosphorus pesticides (DOPs). DOPs are widely used for agricultural and landscape pest control. However, it has been reported that OPs are harmful to human health though food exposure.
Application Notes: The specificity of this antibody for a broad set of O,O-diethyl organophosphorus pesticides (DOPs) was confirmed by indirect competitive ELISA. IC50 of the Fab antibody was 135.7ng/mL. A standard curve showed that the linear detection range is 7.2ng/mL-861.7ng/mL. The Fab fragment detected soluble DOPs by western blot (Chen et al., 2018;PMID: 29734787).
Antibody first published in:
Chen et al. Production of Antigen-Binding Fragment against O,O-Diethyl Organophosphorus Pesticides and Molecular Dynamics Simulations of Antibody Recognition. Int J Mol Sci. 2018 May; 19(5): 1381. PMID:29734787
Note on publication:
The paper describes the generation and characterization of the Fab fragment. The antibody reacts against a broad set of O,O-diethyl organophosphorus pesticides (DOPs). The 3D model of Fab was built via homologous modeling and the interaction between Fab and DOPs was studied via molecular docking and dynamics simulations.