United Kingdom (UK)
Recombinant monoclonal antibody to Digoxigenin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 19-11.
Alternative Name(s) of Target: Digoxigenin; DOG; DIG; ChEBI: 42098; CAS:1672-46-4
Immunogen: The original antibody was generated by immunizing mice with digoxin conjugated to human serum albumin.
Specificity: This antibody is specific for digoxigenin A. Digoxigenin (DIG) is a hydroxy steroid that consists of 5beta-cardanolide, found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata. It is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications.
Application Notes: This antibody can be used for the detection of digoxigenin via ELISA. This antibody was also used for the generation of bispecific antibodies that bind cell-surface targets as well as digoxigenin (Dig) for targeted payload delivery (PMID: 21536919).
Antibody first published in:
Metz et al. Bispecific digoxigenin-binding antibodies for targeted payload delivery. Proc Natl Acad Sci U S A. 2011 May 17; 108(20): 8194–8199. PMID:21536919
Note on publication:
Describes the generation of bispecific antibodies for targeted payload delivery.