United Kingdom (UK)
Recombinant monoclonal antibody to Digoxigenin B. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the human B cell clone Clone 2.
Alternative Name(s) of Target: Digoxigenin; DigB; DOG; ChEBI: 42098; CAS:1672-46-4
Immunogen: The original antibody was isolated from a IgM-derived phage antibody library created from the peripheral blood lymphocytes of 20 non-immunized doners. The specific clone was isolated by panning the library against digoxigenin.
Specificity: This antibody is specific for digoxigenin A. Digoxigenin (DIG) is a hydroxy steroid that consists of 5beta-cardanolide, found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata. It is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications.
Application Notes: The binding specificity of this antibody for digoxigenin was checked using ELISA. This antibody was able to bind digoxigenin coupled by a methylcarbonyl (Dig-Met) or succinyl (Dig-Suc) type linker to BSA. The presence of Dig-dUTP conjugate resulted in competitive inhibition on binding of this antibody to digoxigenin coupled with methylcarbonyl (Dig-Met) liked to BSA (PMID: 9305722).
Antibody first published in:
Dörsam et al. Antibodies to steroids from a small human naive IgM library. FEBS Lett. 1997 Sep 1;414(1):7-13. PMID:9305722
Note on publication:
Describes the generation of antibodies against steroids from a human naive IgM library.