United Kingdom (UK)
Recombinant monoclonal antibody to Do (b). Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Mima-73.
UniProt Accession Number of Target Protein: Q93070
Alternative Name(s) of Target: CD297; Dob; Dombrock glycoprotein; Dombrock (b); 15D6; ADP-ribosyltransferase 4; Ecto-ADP-ribosyltransferase 4; ART4; ARTC4; DO; DO/ART4; DOK1
Immunogen: This antibody was raised by immunising BALB/c mice with human embryonic kidney 293T cells transfected to express high levels of Dombrock b.
Specificity: This antibody is specific for Dombrock b, a blood group antigen carried by ART4. Haemagglutination studies have shown this antibody to react with RBCs from non-human primates, including chimpanzee and orangutan (Mogos et al, 2003).
Application Notes: The specificity of this antibody has been confirmed in haemagglutination studies, where it reacts with Do(a-b+) and Do(a+b+) RBCs, but not with Do(a+b-) RBCs (Grodecka et al, 2012). The epitope recognised by this antibody is resistant to treatment with papain, alpha-chymotrypsin and neuraminidase, and sensitive to treatment with trypsin and DTT (Grodecka et al, 2012). This antibody has been used, as part of a panel of antibodies specific for different epitopes on the Dombrock glycoprotein, in flow cytometric and haemagglutination analysis of human red blood cells (Mayer et al, 2010; Costa et al, 2010).
Antibody first published in:
Grodecka et al. The production, serologic evaluation, and epitope mapping of ten murine monoclonal Dombrock antibodies Immunohematology. 2012;28(4):124-9. PMID:23421541
Note on publication:
Describes the original generation of this antibody, and the characterisation of its antigen specificity.