United Kingdom (UK)
Recombinant monoclonal antibody to Doc2b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N150/21.3.
UniProt Accession Number of Target Protein: P70610
Alternative Name(s) of Target: N150/21.3R; N150/21; Double C2-like domain-containing protein beta; Doc2-beta
Immunogen: This antibody was raised by immunising BALB/c mice with a fusion protein corresponding to amino acids 1-216 (N-terminus) of rat Doc2b.
Specificity: This antibody is specific for the Doc2b protein and it does not show any cross reactivity with the Doc2a protein. Doc2b is a calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes.
Application Notes: This antibody is recommended for the detection and analysis of Doc2b by western blot and immunohistochemistry. For instance, the mouse version of this antibody was used to detect Doc2b by western blot in extracts of RBM and COS cells transiently transfected with GFP-tagged Doc2b or untagged Kv2.1 plasmids. Besides western blot it was also used to detect Doc2b in adult rat hippocampus using immunohistochemistry.
Antibody first published in:
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication:
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.