United Kingdom (UK)
Recombinant monoclonal antibody to E-selectin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 7A9.
UniProt Accession Number of Target Protein: P16581
Alternative Name(s) of Target: CD62E; ELAM-1; ELAM1; Leukocyte-endothelial cell adhesion molecule 2; endothelial leukocyte adhesion molecule-1; CD62 antigen-like family member E; Leukocyte-endothelial cell adhesion molecule 2; SELE
Immunogen: The original antibody was generated by immunizing BALB/c mice with IL-1 activated human endothelial cells.
Specificity: The antibody recognizes the endothelial leukocyte adhesion molecule-1 (ELAM-1) protein as expressed in COS cells.
Application Notes: The F(ab')2 fragment of this antibody showed dose-dependent inhibition of cells activated with IL-1 beta, cells used were human granulocytes. The antibody binds to IL-1 activated human endothelial cells (HEC) but not significantly to normal resting HEC.
Antibody first published in:
Graber et al. T cells bind to cytokine-activated endothelial cells via a novel, inducible sialoglycoprotein and endothelial leukocyte adhesion molecule-1. J Immunol. 1990 Aug 1;145(3):819-30. PMID:1695646
Note on publication:
A study on effect of human rIL-1 beta on confluent, quiescent monolayers of human umbilical vein endothelial cells (HUVEC) was performed.