United Kingdom (UK)
Recombinant monoclonal antibody to E. coli LacI. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9A5.
UniProt Accession Number of Target Protein: P03023
Alternative Name(s) of Target: Lactose operon repressor
Immunogen: The original antibody was generated by immunizing mice against E.coli LacI.
Specificity: The antibody recognizes LacI and recombinant proteins containing the LacI tag. The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. These genes are repressed when lactose is not available to the cell, ensuring that the bacterium only invests energy in the production of machinery necessary for uptake and utilization of lactose when lactose is present. When lactose becomes available, it is converted into allolactose, which inhibits the lac repressor's DNA binding ability. Loss of DNA binding by the lac repressor is required for transcriptional activation of the operon.
Application Notes: This antibody was used for immunohistochemistry on tissue sections from mice transgenic for the R transgene (muscle, skin, hippocampus, cerebellum) (Cronin et al, 2001; PMID: 11410531). The antibody was used to detect the lac repressor protein by western blot analysis (Blevins et al., 2007; PMID: 17220265). The antibody was employed to stain third instar larval salivary glands (Hines et al., 2009; PMID: 19487560). Immunofluorescence staining using the antibody was performed on parental B49.5 lacO integrated cells and B49.5 cells stably expressing lacI-lap2b (Finlan et al., 2008; PMID: 18369458).
Antibody first published in:
Cronin et al. The lac operator-repressor system is functional in the mouse. Genes Dev. 2001 Jun 15;15(12):1506-17. PMID:11410531
Note on publication:
The paper describes the generation and characterization of the antibody.