United Kingdom (UK)
Recombinant monoclonal antibody to Enrofloxacin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody A2.
Alternative Name(s) of Target: Baytril
Immunogen: The original antibody was isolated from a human scFv phage display library by panning against biotinylated enrofloxacin antibiotic.
Specificity: This antibody is a specific binder for enrofloxacin and did not cross-react with other fluoroquinolone antibiotics including structurally similar ciprofloxacin.
Application Notes: This antibody was capable of binding the biotin analogue of enrofloxacin in an ELISA. The binding affinity of the original scFv antibody to enrofloxacin-biotin analogue was reported to be Kd = 2.5 µM. This antibody was used in the development of an ScFv biosensor for qualitative detection of antibiotics in livestock drinking water (PMID: 21142055),
Antibody first published in:
Young Cha et al. Pharmacophore-based strategy for the development of general and specific scFv biosensors for abused antibiotics. Bioconjug Chem. 2011 Jan 19;22(1):88-94. PMID:21142055
Note on publication:
Describes the development of a fluorescent biosensor system using antibodies for detection of fluoroquinolone antibiotics.