United Kingdom (UK)
Recombinant monoclonal antibody to F protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 3B1.
UniProt Accession Number of Target Protein: P03420
Alternative Name(s) of Target: fusion glycoprotein; Fusion glycoprotein F0
Immunogen: A synthetic, fully human antibody library was used for panning against RSV prefusion and post fusion F proteins. The original antibody against RSV F protein was discovered following phage library panning and characterized.
Specificity: The original antibody is specific for post fusion RSV F protein (amino acids D385, F387 and N388). F-protein plays a pivotal role in the pathogenesis of RSV by mediating the fusion between the viral and the host cell membrane. F protein exists in two distinct conformations: the metastable prefusion conformation and the stable post fusion conformation.
Application Notes: The specificity of the Fab and IgG1 format of the antibody was confirmed by ELISA analysis (EC50 value for IgG1 format at 3.6 ng/mL). The binding affinity of the Fab fragment to post fusion form of F was measured by surface plasmon resonance (Kd= 68 nM).
Antibody first published in:
Chen et al. Discovery and Characterization of Phage Display-Derived Human Monoclonal Antibodies against RSV F Glycoprotein PLoS One. 2016; 11(6): e0156798. PMID:27258388
Note on publication:
The paper describes the generation and characterization of the antibody.